Jasmonic Acids Analysis Service

Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of Jasmonic acids (JA) in plants, which can satisfy the needs of academic and industrial study in your lab.

Overview

Jasmonic acids (JA) are a class of lipidic plant hormones, synthesized from linolenic acid present in the chloroplast membrane. They are involved in development, abiotic stress responses and plant-microbes interactions in defence and symbiosis.

In abiotic stress, JA is usually involved in physiological and molecular responses. Physiological responses often include activation of the antioxidant system (superoxide anion radical, peroxidase, NADPH-oxidase), accumulation of amino acids (isoleucine and methionine) and soluble sugars, and regulation of stomatal opening and closing.

The role of jasmonic acid (JA) in plant response to abiotic stressFig1. The role of jasmonic acid (JA) in plant response to abiotic stress (Wang et al., 2020)

JA are also involved in a huge number of plants expanding events, such as primary root growth, leaf senescence, and reproductive development. Moreover, JA is implicated into the regulation of various metabolites production, such as phytoalexins and terpenoids.

Currently, Creative Proteomics presents an LC-MS/MS method for fast and highly sensitive determination of JA. A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. This strategy resulted in dramatic increase in overall sensitivity, allowing successful determination of phytohormones in small (less than 50 mg of fresh weight) tissue samples. The method was completely validated in terms of analyte recovery, sensitivity, linearity and precision.

Why Choose Us?

  • Our method can be applied for the analyses of JA in small tissue samples obtained from any plant species and/or plant part.
  • Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.
  • Send us your sample and receive data in 2-4 weeks.

Our Jasmonic Acids Analysis Service

The workflow of jasmonic acids analysis serviceFig 2. The workflow of jasmonic acids analysis service

Structural details: species level
Mode: MRM
Precision: pmol & mol%
Delivery time: 2-4 weeks
Sample requirements
20 - 50 mg of fresh plant material

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, Creative Proteomics provides reliable, fast and cost-effective JA analysis services. If you have any questions about our JA targeted lipidomics services, please contact us.

References:

  1. Ghorbel, Mouna.; et al. Role of jasmonic acid in plants: the molecular point of view. Plant Cell Reports. 2021, 1-24.
  2. Wang, Jia.; et al. Functions of jasmonic acid in plant regulation and response to abiotic stress. International journal of molecular sciences. 2020, 21.4: 1446.
* Our services can only be used for research purposes and Not for clinical use.

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