Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of CerEOS in skin sample, which can satisfy the needs of academic and industrial study in your lab.
Omega-hydroxy fatty acids [EO] Sphingosine [S] Ceramide (CER[EOS] or EOS) is a subclass of acylceramides. They are composed of sphingosine bases (especially sphingosine) and fatty acids. The fatty acids are omega-hydroxy fatty acids and can be variable length, hydroxylated and contain double bonds. Recent studies have reported that EOS ceramide is very important for the lamellar phase of the epidermal barrier function. It can prevent transdermal water loss and protect it from environmental factors. Skin ceramide imbalance can cause or promote the development of skin conditions, and lower EOS levels are related to atopic dermatitis, psoriasis and dry skin.
CerEOS samples, collected by tape stripping of human skin, were analyzed by highly sensitive LC-MS/MS method in Creative Proteomics. Tape stripping is an almost non-invasive method with the benefits of controlling the sampling depth and collecting comparable sample amounts. This enables collecting a high number of samples and allows screening and biomarker studies, increasing much needed statistical validity of results and conclusions.
Fig1. The protocol workflow of CerEOS targeted lipidomics (Creative Proteomics)
Structural details: species level
Precision: pmol & mol%
Variants identified: >10
Delivery time: 3-6 weeks
Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective CerEOS analysis services. If you have any questions about our CerEOS targeted lipidomics services, please contact us.