Fatty Acid Analysis Service

Fatty acids are a class of aliphatic carboxylic acid compounds containing long-chain hydrocarbons, usually in the form of esters in various lipid components such as neutral fats, phospholipids and glycolipids. According to the carbon chain length, fatty acids can be divided into short-chain fatty acids (2-4 carbon atoms), medium-chain fatty acids (6-12 carbon atoms) and long-chain fatty acids (more than 14 carbon atoms). According to hydrocarbon chain saturation and unsaturation, fatty acids can be divided into saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids. Palmitic acid, stearic acid, and arachidic acid are saturated fatty acids. Myristic acid, palmitoleic acid, oleic acid, erucic acid, etc. belong to monounsaturated fatty acids. Linoleic acid, linolenic acid, arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid are polyunsaturated fatty acids.

Fatty acids are associated with cardiovascular diseases, hypertension, diabetes, hyperlipidemia, obesity and other metabolic diseases. It also plays a role in anti-tumor, anti-depression, inflammation, promoting brain and retinal development, and promoting bone health.

In addition, fatty acid β oxidation is the main way of fatty acid decomposition in organisms, which provides a lot of energy for the body. Ketone bodies, the intermediate metabolites of fatty acids, including acetoacetic acid, β-hydroxybutyric acid and acetone, are also important for life activities.

Fatty acid content in human body can be regulated through dietary intake. The role of fatty acids in the body depends on the structure. We can make a detailed analysis of the composition of fatty acids contained in foods.

Creative Proteomics has established a fatty acid analysis platform, using LC-MS/MS and GC-MS/MS technologies to achieve efficient and accurate analysis of fatty acid composition and quantitative analysis of fatty acids.

Our services can be used in medicinal and oil plant composition research, metabolite kinetic analysis, metabolic pathway analysis, lipid metabolism research, disease diagnosis, food and oil standard testing, nutrition and development research, etc.

List of detectable fatty acid at Creative Proteomics

Technology platform

  • High performance liquid chromatography (HPLC)
  • Gas chromatography-mass spectrometry (GC-MS)
  • Liquid chromatography-mass spectrometry (LC-MS/MS)

A typical workflow of mass spectrometry-based lipidomicsA typical workflow of mass spectrometry-based lipidomics (Wang et al., 2019)

Sample requirements

  • Blood/plasma: ≥ 500 ul/sample
  • Urine: ≥ 1 ml/sample
  • Animal tissue: ≥ 200 mg/sample
  • Plant tissue: ≥ 200 mg/sample
  • Microorganism ≥ 200 mg/sample

If you want to analyze other samples, please contact our experts for consultation.

Deliverables

  • Experimental procedure
  • Parameters of gas chromatography, liquid chromatography, mass spectrometry
  • MS raw data files and MS data quality checks
  • Spectrum of fatty acid analysis
  • Custom analysis report

Delivery time: 2-4 weeks

Creative Proteomics has many years of experience in lipidomics analysis services, and enjoys a good reputation in supporting the detection and quantification of various lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, we provide reliable, fast and cost-effective fatty acid analysis services. If you have other substances you want to test or other questions, please contact us.

Reference:

  1. Wang, Jianing, Chunyan Wang, and Xianlin Han. "Tutorial on lipidomics." Analytica chimica acta 1061 (2019): 28-41.
* Our services can only be used for research purposes and Not for clinical use.

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