Lipidomics for Alzheimer's Disease Research
Alzheimer's Disease (AD) is the most common dementia among all the clinically recognized dementias in the aging population, which is characterized by a progressive loss of short-term memory accompanied by a gradual loss of cognitive functions. It is a complex neurodegenerative disease that lacks early diagnosis and effective treatment options. Biomarker identification and analysis will be particularly important in the management of AD.
As lipids can be linked to the hallmarks of AD, an in-depth analysis of lipid composition changes in AD might provide a different perspective on AD neuropathology and potentially different AD biomarkers. Detailed regional lipidomic analyses of AD brain and cerebrospinal fluid samples have provided in-depth comparisons at different disease stages of AD and may identify lipid biomarkers for early diagnosis of AD.
With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high sensitivity, Creative Proteomics provides customized lipidomics solutions for Alzheimer's disease research.
Featured services
Strategies for Lipidomic Analysis of Alzheimer's Diseases
Creative Proteomics designs experimental protocols based on customer needs and selects different technical methods to improve assay efficiency and ensure the accuracy and reproducibility of results.
Based on LC-MS/MS technology to simultaneously analyze hundreds of different lipids in biological samples to obtain more information. Through bioinformatics data analysis, we can systematically elucidate the variation at the lipidome level and the regulatory mechanisms in the experimental group compared to the control group from a lipid perspective.
Targeting specific lipid molecules and their metabolites in samples. Based on high-resolution mass spectrometry and isotope internal standard, we use parallel reaction monitoring (PRM) targeted analysis technology, which can realize the specific acquisition of signals of multiple lipid molecules (such as dozens of target lipid molecules) at the same time, and obtain their absolute contents to meet the needs of targeted detection and verification of target lipids. Using Orbitrap mass analyzer and ultra-high resolution mass spectrometry, high quality data can be obtained. Sensitivity up to ppm level and linearity range up to 5-6 orders of magnitude.
Technology Platform for Lipidomics Service
TriVersa Nanomate nanoelectrospray ion source+ Orbitrap instruments.
Liquid chromatography or gas chromatography hyphenated to triple quadrupole mass spectrometers for targeted lipidomics.
Shimadzu Nexera X2 U-HPLC System+ AB Sciex 6500 QTRAP.
Thermo Scientific TSQ 9000 triple quadrupole GC-MS/MS system.
A detailed report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameter, raw data and data reports with figures, ready to be published.
Schematic diagram of the parallel metabolomic and lipidomic workflows (Bhawal et al., 2021)
Advantages of Lipidomics Services
- Experienced in sample pre-processing and can handle a wide range of tissue cell samples
- Multi-isotope internal standard calibration, including QC and other multiple quality control systems to make the quantitative more accurate
- Suitable for complex matrices, more metabolite and metabolic network information can be obtained in a single test
- Professional data analysis, able to perform PCA, OPLS-DA, T-test and other analysis on the data
How we work in 7 easy steps
If you have any questions about our lipidomics services for Alzheimer's disease research, please contact us.
Reference:
- Bhawal, R., Fu, Q., et al. (2021). Serum Metabolomic and Lipidomic Profiling Reveals Novel Biomarkers of Efficacy for Benfotiamine in Alzheimer's Disease. International Journal of Molecular Sciences, 22(24), 13188.
* Our services can only be used for research purposes and Not for clinical use.
