Microorganisms Lipidomics in Drug Research
Lipomics can provide the changes of microbial lipids and related enzymes, which is of great significance for drug research in the field of microbiology. Here are some related studys.
- Candida albicans-associated bloodstream infections are linked to the ability of this yeast to form biofilms. A study used lipidomics to compare the lipid profiles of C. albicans biofilms and planktonic cells, in early and mature developmental phases;
- The results showed that significant differences exist in lipid composition in both developmental phases. Biofilms contained higher levels of phospholipid and sphingolipids than planktonic cells;
- Inhibition of the biosynthetic pathway for sphingolipid by myriocin or aureobasidin A, and disruption of the gene encoding inositolphosphotransferase (Ipt1p), abrogated the ability of C. albicans to form biofilms.
- Drug Resistance and Slower Response to Antibiotics Therapy
- Mycobacterium tuberculosis employs several strategies to combat and adapt to adverse conditions encountered inside the host. The non-replicative dormant state of the bacterium is linked to drug resistance and slower response to anti-tubercular therapy.
- It is known that alterations in lipid content allow dormant bacteria to acclimatize to cellular stress. Untargeted lipidomic analysis profiled an enhanced degradation of cell wall-associated and cytoplasmic lipids during dormancy, and their gradual restoration during reactivation.
- Research on the Mechanism of Drug
- Positive-strand RNA viruses use the host cell membrane to complete their genome replication. The specific mechanism is not understood.
- Transcriptomics studies have shown that during infection or replication of the virus, genes involved in lipid metabolism in host cells are up-regulated. Lipidomics studies have shown that host phospholipids change significantly during viral RNA replication, especially phosphatidylcholine (PC) , Regulating PC synthesis may be a new anti-viral method.
Creative Proteomics provides reliable, rapid and cost-effective microorganisms untargeted lipidomics based on LC-MS or shot-gun methods for drug research.
Creative Proteomics routinely cover a rich variety of sample types, including bacteria, virus, fungus and parasites for our customer. Still, our services require only minimal sample amounts per analysis.
If you have any questions about sample collection, please contact us.
Fig1. The protocol workflow of microorganisms lipidomics in drug research (Creative Proteomics)
What Creative Proteomics Provides for Our Customers?
- Send us your sample and receive data in 2-4 weeks, lower sample demand;
- Professional and customized experimental design to meet your research requirements;
- Creative Proteomics offers a report including experiment procedures, parameters of liquid chromatography and mass spectrometer, MS raw data files, MS data quality checks, metabolites quantification data and bioinformatics analysis (PCA, KEGG, etc).
If you have any questions about our microorganisms untargeted lipidomics services, welcome to contact us.
- Lattif, A. A.; et al. Lipidomics of Candida albicans biofilms reveals phase-dependent production of phospholipid molecular classes and role for lipid rafts in biofilm formation. Microbiology. 2011, 157(Pt 11), 3232.
- Raghunandanan, S.; et al. Comparative label-free lipidomic analysis of Mycobacterium tuberculosis during dormancy and reactivation. Sci Rep. 2019, 9, 3660.
- Castorena KM.; et al. Complementary transcriptomic, lipidomic, and targeted functional genetic analyses in cultured Drosophila cells highlight the role of glycerophospholipid metabolism in Flock House virus RNA replication. BMC Genomics. 2010 Mar 17;11:183.
* Our services can only be used for research purposes and Not for clinical use.