Lipoxins Analysis Service

Lipoxins are one such anti-inflammatory, pro-resolving molecule that is secreted by immune cells such as neutrophils and macrophages. Lipoxins are endogenous anti-inflammatory, pro-resolving molecules that play a vital role in reducing excessive tissue injury and chronic inflammation.

Lipoxins regulate components of both the innate and adaptive immune systems including neutrophils, macrophages, T-, and B-cells. Lipoxins also modulate levels of various transcription factors such as nuclear factor κB, activator protein-1, nerve growth factor-regulated factor 1A binding protein 1, and peroxisome proliferator activated receptor γ and control the expression of many inflammatory genes.

Lipoxins play an important role in clinical research to treat a wide range of diseases like inflammatory disorders, renal fibrosis, cerebral ischemia, and cancer.

Effect of lipoxins on cytoplasmic signaling cascades and transcription factorsFig 1. Effect of lipoxins on cytoplasmic signaling cascades and transcription factors (Chandrasekharan JA et al., 2015)

Creative Proteomics provides comprehensive lipoxins targeted lipidomics services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Our Lipoxins Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for lipoxins analysis service (Triple Quad™ 6500 LC-MS/MS System).

The workflow of lipoxins analysis service.Fig 2. The workflow of lipoxins analysis service.

Sample Requirements

We routinely cover a rich variety of sample types, including cells, conditioned medium, exosome, serum, plasmafor our customer.

Extraction of lipids should be performed right after sample collection steps, otherwise, samples should be stored at -80°C, and shipped with dry ice.

After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.

Why Choose Us?

  • Fast turnaround time: 1-4 weeks.
  • High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Bioinformatics analysis: we provide data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
  • Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

If you have any questions about our lipoxins analysis services, please contact us.

Reference:

  1. Chandrasekharan JA.; Sharma-Walia N. Lipoxins: nature's way to resolve inflammation. J Inflamm Res. 2015, 8:181-192.
* Our services can only be used for research purposes and Not for clinical use.

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