Phosphatidylglycerol Analysis Service

Phosphatidylglycerol (PG) is a ubiquitous anionic phospholipid that constitutes the biological membranes of animals, plants and microorganisms. It is known that PG is synthesized by a common pathway in prokaryotes and eukaryotes.

PG is the main phospholipid component of the chloroplast membrane, accounting for about 10% of the total membrane lipid. The fatty acid composition of PG is closely related to the cold resistance of plants. Glycerol-3-phosphate acyltransferase plays an important role in the fatty acid composition of PG.

PG are found as major phospholipid components of cell membranes in bacteria, representing 20–25% of total lipid phosphorus in most Gram-negative bacteria with a double (outer and inner) phospholipid envelop, where it is restricted within the inner membrane.

In Gram-positive strains (with a single phospholipid bilayer coated with peptidoglycans) it can be as high as 60% and even found as the major phospholipid besides sugar derivatives of diacylglycerol.

In contrast, PG is a minor phospholipid component in mammals, found at only trace amounts in tissues and fluids except in lung surfactant. But it also play an important role in human disease.

Creative Proteomics provides you with efficient and accurate qualitative and quantitative detection services and the fatty acid composition analysis of phosphatidylglycerol through advanced chromatography and mass spectrometry platforms.

Our Phosphatidylglycerol Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for phosphatidylglycerol analysis service (Triple Quad™ 6500 LC-MS/MS System).

The workflow of phosphatidylglycerol analysis serviceFig 1. The workflow of phosphatidylglycerol analysis service

Sample Requirements

We routinely cover a rich variety of sample types, including animal and plant tissues, microorganisms, serum, cells, cell medium, et al. for our customer.

The samples should be stored at -80°C and shipped on dry ice.

Why Choose Us?

  • Fast turnaround time: 1-4 weeks.
  • High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.
  • We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.

If you have any questions about our phosphatidylglycerol detection service, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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