Ether-linked phosphatidylcholine (PC O-) is a type of glycerophospholipid. Their structure consists of a glycerol backbone linking two fatty acids and a phosphocholine molecule. One of the fatty acids is bonded to the glycerol backbone through an ether bond.
Ether-linked phosphatidylcholine showed significantly lower levels in patients with multiple sclerosis and patients in both remission and relapse of multiple sclerosis.
It has been found that levels in cancer are elevated, especially in aggressive forms. In addition, PC O- lipid is an important membrane component of neutrophils, which are the most abundant type of human leukocytes.
Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of ether-linked phosphatidylcholines in different sample types, which can satisfy the needs of academic and industrial study in your lab.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for ether-linked phosphatidylcholine analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of ether-linked phosphatidylcholine analysis service
We routinely cover a rich variety of sample types, including cells, serum, and plasma, et al.
Each sample should preferably contain 5x106 cells, preferably 1x107 cells. If your cells are primary cells, we need 2x106 cells.
According to different experimental schemes, we usually need 100-200 ul plasma or serum for our lipidomics services.
The samples are shipped on dry ice. The tube should be clearly marked with the sample identifier. When shipping samples, please include all available sample information.
Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics. If you have any questions about our ether-linked phosphatidylcholine detection service, please contact us.