Phosphatidylcholine (PC) is the most abundant phospholipid of all mammalian cell types and subcellular organelles. In general, PC comprises 40–50% of total cellular phospholipids, although different cell types, individual organelles and even the two leaflets of organelle membranes contain distinct phospholipid compositions.
Since its discovery in the 19th century, PC has been regarded primarily as a structural lipid. However, more recent evidence strongly suggests that PC has other roles. 1) PC can help boost cognitive function; 2) PC can aid in liver repair; 3) PC can help protect against medication side effects; 4) PC can help ease symptoms of ulcerative colitis; 5) PC can promote lipolysis; 6) PC can help dissolve gallstones.
Therefore, identification the level of PC can offer novel biomarkers for specific disease, also provide new insights for us to deeply understand the pathogenic mechanism of diseases.
Creative Proteomics uses liquid chromatography mass spectrometry (LC-MS) method to identify the samples, which can effectively and accurately detect the changes in the content of phosphatidylcholine.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: we use liquid chromatography coupled with triple quadrupole mass spectrometers for phosphatidylcholine analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 1. The workflow of phosphatidylcholine analysis service.
We can accept a variety of sample types, including animal and plant tissues, cells, bacteria, food, etc.
Extraction of lipids should be performed right after sample collection steps, otherwise, samples should be stored at -80°C, and shipped with dry ice.
After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.
If you have any questions about our glycerophosphocholines targeted lipidomics services, please contact us.