Short Chain Fatty Acids Analysis Service

Short-chain fatty acids (SCFA), also known as volatile fatty acids (VFAs), refer to organic fatty acids with 2-6 carbon atoms. SCFAs mainly include acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, caproic acid and isohexanoic acid.

SCFAs can participate in the energy supply of intestinal epithelial cells, and gradually become signal molecules that regulate diet, intestinal microbes and the host. In the intestine, SCFAs can regulate intestinal immunity, glucose homeostasis, blood lipids, and reduce the risk of obesity and diabetes.

As the main energy substrate, SCFAs have anti-inflammatory and anti-cancer effects. SCFAs can affect chronic diseases through a variety of mechanisms, play an important role in maintaining the metabolic homeostasis of colonic epithelial cells, and protect the colon from external damage.

Creative Proteomics can provide you with one-stop high-sensitivity quantitative analysis of SCFAs, including SCFAs with C2-C12 carbon chain lengths of various biological materials (feces and serum, etc.).

Gut microbial short-chain fatty acids and the risk of diabetesFig1. Gut microbial short-chain fatty acids and the risk of diabetes (Lau, W.L.;2019)

Our Short Chain Fatty Acids Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: we use gas chromatography hyphenated to triple quadrupole mass spectrometers for short chain fatty acids targeted lipidomics (Thermo Scientific TSQ 9000 triple quadrupole GC-MS/MS system).

Determination of short-chain fatty acids by GC-MSDetermination of short-chain fatty acids by GC-MS (Wang et al., 2020)

Sample Requirements

  • Serum or plasma: ≥ 100 ul/sample
  • Stool and intestinal contents: ≥ 100 mg/sample
  • Saliva: ≥ 100 mg/sample

Quick-freeze in liquid nitrogen and store at -80°C, and send it on dry ice.
If you provide other samples or want to know the specific sample size, please contact us.

Why Choose Us?

  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Fast turnaround time: 1-4 weeks.
  • Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

If you have any questions about our short chain fatty acids analysis services, please contact us.


  1. Lau, W.L.; Vaziri, N.D. Gut microbial short-chain fatty acids and the risk of diabetes. Nat Rev Nephrol.2019, 15, 389–390.
  2. Wang, R., et al. (2020). A fast and accurate way to determine short chain fatty acids in human serum by GC–MS and their distribution in children with digestive diseases. Chromatographia, 83(2), 273-286.
* Our services can only be used for research purposes and Not for clinical use.




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