Ganglioside Analysis Service

Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of ganglioside in different sample types, which can satisfy the needs of academic and industrial study in your lab.

Overview

Ganglioside Targeted Lipidomics

A ganglioside is a molecule composed of a glycosphingolipid with one or more sialic acids (e.g. n-acetylneuraminic acid, NANA) linked on the sugar chain. Gangliosides are ubiquitously found in tissues and body fluids, and are more abundantly expressed in the nervous system. In cells, gangliosides are primarily, but not exclusively, localized in the outer leaflets of plasma membranes. On the cell surface, gangliosides are involved in cell-cell recognition and adhesion and signal transduction within specific cell surface microdomains, termed caveolae, lipid rafts, or glycosphingolipid-enriched microdomains, with other membrane components such as sphingomyelin and cholesterol. Evidence is accumulating that gangliosides are colocalized in the microdomain structures with signaling molecules and adhesion molecules. In addition to cell plasma membranes, gangliosides have been shown to be present on nuclear membranes, and they have recently been proposed to play important roles in modulating intracellular and intranuclear calcium homeostasis and the ensuing cellular functions.

The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of ganglioside in different sample types has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.

Advantages of Our Ganglioside Analysis Service

  • One-stop analysis services, from sample preparation, mass spectrometry, data processing, to the identification and quantification of lipids.
  • Creative Proteomics provide excellent lipidomics services with low prices and fast turnaround times. Send us your samples and recive results in 2-4 weeks.
  • High resolution, high mass accuracy instrumentation to provide credible results.

List of Detectable Ganglioside at Creative Proteomics

GM1 34:1;2GM1GM1 36:1;2GM2 38:1;2GM2
GM2 40:1;2GM3 38:1;2GM3GM3 32:1;2GM4 36:1;2
GM4GM4 42:1;2GD1GD1 34:1;2GD1 38:1;2
GD2GD3GT1GT2GT3

The Technical Process of Our Ganglioside Analysis Service

The workflow of globoside analysis serviceFig 2. The workflow of globoside analysis service

Structural details: species level
Precision: pmol & mol%
Variants identified: >50
Delivery time: 2-4 weeks
Sample requirements
Blood/plasma: 500 ul/sample
Cells: 1x107/sample

  • A minimum of 3 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
  • Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective ganglioside analysis services. If you have any questions about our ganglioside targeted lipidomics services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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