Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of ganglioside in different sample types, which can satisfy the needs of academic and industrial study in your lab.
A ganglioside is a molecule composed of a glycosphingolipid with one or more sialic acids (e.g. n-acetylneuraminic acid, NANA) linked on the sugar chain. Gangliosides are ubiquitously found in tissues and body fluids, and are more abundantly expressed in the nervous system. In cells, gangliosides are primarily, but not exclusively, localized in the outer leaflets of plasma membranes. On the cell surface, gangliosides are involved in cell-cell recognition and adhesion and signal transduction within specific cell surface microdomains, termed caveolae, lipid rafts, or glycosphingolipid-enriched microdomains, with other membrane components such as sphingomyelin and cholesterol. Evidence is accumulating that gangliosides are colocalized in the microdomain structures with signaling molecules and adhesion molecules. In addition to cell plasma membranes, gangliosides have been shown to be present on nuclear membranes, and they have recently been proposed to play important roles in modulating intracellular and intranuclear calcium homeostasis and the ensuing cellular functions.
The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of ganglioside in different sample types has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.
|GM1 34:1;2||GM1||GM1 36:1;2||GM2 38:1;2||GM2|
|GM2 40:1;2||GM3 38:1;2||GM3||GM3 32:1;2||GM4 36:1;2|
|GM4||GM4 42:1;2||GD1||GD1 34:1;2||GD1 38:1;2|
Fig 2. The workflow of globoside analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: >50
Delivery time: 2-4 weeks
Blood/plasma: 500 ul/sample
Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective ganglioside analysis services. If you have any questions about our ganglioside targeted lipidomics services, please contact us.