Phospholipid Fatty Acids Analysis Service

Phospholipids fatty acids (PLFAs) are present in the cell membranes of living microorganisms, are widely distributed, relatively constant in content, and sensitive to changes in environmental factors. PLFAs can be used to identify microbial species and microbial taxa with high accuracy, stability and sensitivity, and are considered to be one of the potential biomarkers.

Phospholipids are important components of cell membranes of almost all organisms, and different groups of microorganisms can synthesize different PLFAs through different biochemical pathways.The content of phospholipids in cells is constant under natural conditions (normal physiological conditions) and is structurally diverse and microbially specific. The presence and abundance of PLFAs can reveal the characteristics of specific microbial species or microbial taxa and represent a microbial fingerprinting of microbial community structure. The characteristic fingerprint profiles of fatty acid biomarkers (PLFAs) of different microbial groups are different and diverse on the basis of high specificity, which can be used as biomarkers for different groups in microbial communities.

In addition, PLFA profiling methods can be used to detect the nutritional status and metabolic activity of microorganisms in environmental samples, etc. Adding the radioactive matrix to the sample before lipid extraction to count and label microorganisms can be used to determine the metabolic activity.

Creative Proteomcis has an LC-MS/MS system capable of performing PLFA profiling to identify and quantitatively characterize microbial communities, helping to elucidate changes in the structure of microbial communities in samples and providing relevant information for further research.

Our Phospholipid Fatty Acids Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for phospholipid fatty acids analysis service (Triple Quad™ 6500 LC-MS/MS System).

The workflow of phospholipid fatty acidss analysis service.Fig 1. The workflow of phospholipid fatty acidss analysis service.

Sample Preparation

We can accept a variety of environmental samples, including soil microorganisms, sediments, marine microorganisms, etc. If you want to provide other samples, please contact our technicians for specific sample sizes.

  • Serum/plasma and other homogeneous liquid samples: ≥ 100ul/sample
  • Cells/microorganisms: ≥ 10*7/sample

Store at -80°C and send with dry ice.

Why Choose Us?

  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • High sensitivity: High-sensitivity mass spectrometer is used to effectively detect low-level metabolites in the sample.
  • Fast turnaround time: 1-4 weeks.
  • PLFA spectral analysis: multivariate statistical methods are used, including principalcomponent analyses (PCA), partial least squares identification (D-PLS), and standard discriminant analysis (RDA).
  • Detailed report: Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

If you have any questions about our phospholipid fatty acids analysis services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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